Spotting in general
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Spotting is a production technique where tiny droplets of liquid samples are precisely deposited onto a solid surface like foils, glass slides, or microplates. It has multiple applications for molecular biology, biochemistry, and diagnostics to deposit specific molecules like DNA, RNA, proteins, or antibodies. Alternatively, coatings can also be applied on a pre-determined location.
The process often relies on specialized tools, such as robotic spotters or pipettes. This is needed to obtain the required accuracy and reproducibility, both in volume and placement. Spots are typically small (often nanoliters or picoliters in volume).
Spotting ensures uniformity, which is critical for comparing results across multiple samples or experiments. Overall, spotting enables efficient, sensitive, and scalable droplet deposition, making it essential for research and medical diagnostics.
